IL18R1 Protein (AA 20-326) (His tag)

Catalog No. ABIN7274982

This Recombinant IL18R1 protein is produced in HEK-293 Cells.

Quick Overview for IL18R1 Protein (AA 20-326) (His tag) (ABIN7274982)

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-326

Purification tag / Conjugate: This IL18R1 protein is labelled with His tag.

Purpose: Mouse IL-18 R1/CD218a Protein

Sequence: Lys20-Gly326

Characteristics: Recombinant Mouse IL-18 R1/CD218a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys20-Gly326.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.30 uM as determined in SPR assay (Biacore T200).,Mouse IL-18 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse IL-18 R1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL18R1 (Interleukin 18 Receptor 1 (IL18R1))

Alternative Name: IL-18 R1

Background: Interleukin-18 (IL-18) is a member of the interleukin-1 family of cytokines produced constitutively by different cell types and by adipose tissue. Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naive T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation.

Molecular Weight: 36.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Bis-Tris PAGE result.

UniProt: Q61098