IL17C Protein (AA 19-197) (His-Avi Tag)

Catalog No. ABIN7274998

This Recombinant IL17C protein is expressed in HEK-293 Cells.

Quick Overview for IL17C Protein (AA 19-197) (His-Avi Tag) (ABIN7274998)

Target: IL17C (Interleukin 17C (IL17C))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 19-197

Purification tag / Conjugate: This IL17C protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human IL-17C Protein

Sequence: His19-Val197

Characteristics: Recombinant Human IL-17C Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains His19-Val197.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-17R alpha, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-17C, His Tag-with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).,Immobilized Human IL-17C, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-17RE, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA (QC Test).,Human IL-17C on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in 50 mM MES, 150 mM NaCl (PH 6.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in 50 mM MES, 150 mM NaCl (PH 6.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL17C (Interleukin 17C (IL17C))

Alternative Name: IL-17C

Background: Interleukin-17C (IL-17C) is a 15-20 kDa glycosylated cytokine that plays an important role in mucosal immunity and chronic inflammation. The six IL-17 cytokines (IL-17A-F) are encoded by separate genes but adopt a conserved cystine knot fold. IL-17C is Cytokine that plays a crucial role in innate immunity of the epithelium, including to intestinal bacterial pathogens, in an autocrine manner. Stimulates the production of antibacterial peptides and proinflammatory molecules for host defense by signaling through the NF-kappa-B and MAPK pathways. Acts synergically with IL22 in inducing the expression of antibacterial peptides, including S100A8, S100A9, REG3A and REG3G.

Molecular Weight: 22.6 kDa. Due to glycosylation, the protein migrates to 25-28 kDa based on Bis-Tris PAGE result.

UniProt: Q9P0M4

Pathways: Cellular Response to Molecule of Bacterial Origin