IL23R Protein (AA 24-355) (His tag)

Catalog No. ABIN7275015

This Recombinant IL23R protein is produced in HEK-293 Cells.

Quick Overview for IL23R Protein (AA 24-355) (His tag) (ABIN7275015)

Target: IL23R (Interleukin 23 Receptor (IL23R))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-355

Purification tag / Conjugate: This IL23R protein is labelled with His tag.

Purpose: Human IL-23R Protein

Sequence: Gly24-Gly355

Characteristics: Recombinant Human IL-23R Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly355.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-23R, His Tag immobilized on CM5 Chip can bind Human IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 5.72 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-23R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-23 alpha&IL-12 beta, His Tag with the EC50 of 2.75ug/ml determined by ELISA (QC Test).,Human IL-23R on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human IL-23R is greater than 95% and the molecular weight of this protein is around 55-70 kDa as determined by SEC-MALS.,The purity of Human IL-23R is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL23R (Interleukin 23 Receptor (IL23R))

Alternative Name: IL-23R

Background: Interleukin-23 (IL-23), a member of the IL-12 family of cytokines, is a heterodimeric cytokine. It is composed of subunits p40 (shared with IL-12) and p19 (an IL-12 p35-related subunit) and is secreted by several types of immune cells, such as natural killer cells and dendritic cells. The IL-23 receptor is composed of the subunit IL-12Rβ1 and the IL-23-specific subunit IL-23R. The binding of IL-23 to its specific cell surface receptor regulates a number of functions, including proliferation and differentiation of cells and secretion of cell factors.

Molecular Weight: 39.15 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

UniProt: Q5VWK5

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation