IL6RA Protein (AA 20-365) (hFc Tag)

Catalog No. ABIN7275024

This Recombinant IL6RA protein is produced in HEK-293 Cells.

Quick Overview for IL6RA Protein (AA 20-365) (hFc Tag) (ABIN7275024)

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-365

Purification tag / Conjugate: This IL6RA protein is labelled with hFc Tag.

Purpose: Human IL-6 R alpha/CD126 Protein

Sequence: Leu20-Pro365

Characteristics: Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu20-Pro365.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-6 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-6 R alpha, hFc Tag with the EC50 6.0ng/ml determined by ELISA (QC Test).,Immobilized Human IL-6 R alpha, hFc Tag at 2ug/ml (100ul/well) on the Human IL-6, No Tag precoated plate (5ug/ml). Dose response curve for Human gp130, His Tag with the EC50 of 49.6ng/ml determined by ELISA.,Human IL-6 R alpha on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Human IL-6 R alpha, hFc Tag on ProA-Biosensor can bind Human IL-6, No Tag with an affinity constant of 16.50 nM as determined in BLI assay (Gator® Prime).,The purity of Human IL-6 R alpha is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Alternative Name: IL-6 R alpha

Background: Serum levels of interleukin-6 (IL-6) are increased in patients with type 2 diabetes (T2D). IL-6 exerts its pleiotropic effects via the IL-6 α-receptor (IL-6R), which exists in membrane-bound and soluble (sIL-6R) forms and activates cells via the β-receptor glycoprotein 130 (gp130).

Molecular Weight: 65.3 kDa. Due to glycosylation, the protein migrates to 85-110 kDa based on Bis-Tris PAGE result.

UniProt: P08887

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints