IL5RA Protein (AA 21-335) (His-Avi Tag)
Quick Overview for IL5RA Protein (AA 21-335) (His-Avi Tag) (ABIN7275034)
Target
See all IL5RA ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 21-335
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Purification tag / Conjugate
- This IL5RA protein is labelled with His-Avi Tag.
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Purpose
- Human IL-5 R alpha/CD125 Protein
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Sequence
- Asp21-Glu335
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Characteristics
- Recombinant Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp21-Glu335.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- The affinity constant of 4.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 5 Receptor, alpha (IL5RA) (AA 21-335) protein (His-Avi Tag,Biotin)
IL5RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 Receptor, alpha (IL5RA) (AA 18-339) protein (His tag)IL5RA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-335) protein (Fc Tag)IL5RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-338) protein (His tag)IL5RA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-338) protein (Fc Tag)IL5RA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 Receptor, alpha (IL5RA) (Active) protein (His tag)IL5RA Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 5 Receptor, alpha (IL5RA) (Active) protein (His tag)IL5RA Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 5 Receptor, alpha (IL5RA) (AA 18-339) protein (His tag)IL5RA Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 90% by SDS-PAGE SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- IL5RA (Interleukin 5 Receptor, alpha (IL5RA))
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Alternative Name
- IL-5 R alpha
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Background
- Interleukin-5 Receptor alpha (IL-5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
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Molecular Weight
- 38.7 kDa. Due to glycosylation, the protein migrates to 50-68 kDa based on Tris-Bis PAGE result.
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UniProt
- Q01344-1
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Pathways
- JAK-STAT Signaling
Target
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