IL5RA Protein (AA 21-335) (His-Avi Tag,Biotin)
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- Target See all IL5RA Proteins
- IL5RA (Interleukin 5 Receptor, alpha (IL5RA))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 21-335
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This IL5RA protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human IL-5 R alpha/CD125 Protein
- Sequence
- Asp21-Glu335
- Characteristics
- Recombinant Biotinylated Human IL-5 R alpha/CD125 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp21-Glu335.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human IL-5, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
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Interleukin 5 Receptor, alpha (IL5RA) (AA 18-339) protein (His tag)
IL5RA Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-335) protein (Fc Tag)IL5RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-335) protein (His-Avi Tag)IL5RA Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 5 Receptor, alpha (IL5RA) (Active) protein (His tag)IL5RA Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 5 Receptor, alpha (IL5RA) protein (His tag)IL5RA Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 5 Receptor, alpha (IL5RA) (Active) protein (His tag)IL5RA Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-342) protein (His tag)Crystallography grade IL5RA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 5 Receptor, alpha (IL5RA) (AA 18-339) protein (His tag)Crystallography grade IL5RA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 5 Receptor, alpha (IL5RA) (AA 21-420) protein (rho-1D4 tag)Crystallography grade IL5RA Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Interleukin 5 Receptor, alpha (IL5RA) (AA 18-415) protein (rho-1D4 tag)Crystallography grade IL5RA Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- IL5RA (Interleukin 5 Receptor, alpha (IL5RA))
- Alternative Name
- IL-5 R alpha (IL5RA Products)
- Synonyms
- MGC86517 Protein, IL5RA Protein, il5r Protein, cdw125 Protein, hsil5r3 Protein, gpIL-5ra Protein, CD125 Protein, CDw125 Protein, HSIL5R3 Protein, IL5R Protein, Il5r Protein, interleukin 5 receptor subunit alpha L homeolog Protein, interleukin 5 receptor subunit alpha Protein, interleukin 5 receptor, alpha Protein, il5ra.L Protein, IL5RA Protein, il5ra Protein, Il5ra Protein
- Background
- Interleukin-5 Receptor alpha (IL-5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
- Molecular Weight
- 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
- Pathways
- JAK-STAT Signaling
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