IL6RA Protein (AA 20-365) (His-Avi Tag)

Catalog No. ABIN7275036

This Recombinant IL6RA protein is produced in HEK-293 Cells.

Quick Overview for IL6RA Protein (AA 20-365) (His-Avi Tag) (ABIN7275036)

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-365

Purification tag / Conjugate: This IL6RA protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human IL-6 R alpha/CD126 Protein

Sequence: Leu20-Pro365

Characteristics: Recombinant Human IL-6 R alpha/CD126 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu20-Pro365.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human IL-6 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-6, No Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).,Immobilized Human IL-6, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-6 R alpha, His Tag with the EC50 of 0.21ug/ml determined by ELISA (QC Test).,Human IL-6 R alpha on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,Loaded Human IL-6 R alpha, His Tag on Anti-His-Biosensor can bind Human IL-6, No Tag with an affinity constant of 30.80 nM as determined in BLI assay (Gator® Prime).,The purity of- Human IL-6 R alpha is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL6RA (Interleukin 6 Receptor, alpha (IL6RA))

Alternative Name: IL-6R alpha

Background: The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6R alpha) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6R alpha and gp130 have been cloned, sequenced, and expressed.

Molecular Weight: 41.4 kDa. Due to glycosylation, the protein migrates to 65-78 kDa based on Bis-Tris PAGE result.

UniProt: P08887

Pathways: JAK-STAT Signaling, Autophagy, Growth Factor Binding, Cancer Immune Checkpoints