IL13 Receptor alpha 1 Protein (AA 27-343) (His-Avi Tag,Biotin)

Catalog No. ABIN7275038

Recombinant IL13 Receptor alpha 1 protein expressed in HEK-293 Cells.

Quick Overview for IL13 Receptor alpha 1 Protein (AA 27-343) (His-Avi Tag,Biotin) (ABIN7275038)

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-343

Purification tag / Conjugate: This IL13 Receptor alpha 1 protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human IL-13Ra1 Protein

Sequence: Ala27-Thr343

Characteristics: Recombinant Biotinylated Human IL-13Ra1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Thr343.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-13, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-13Ra1, His Tag with the EC50 of 2.8μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: IL13 Receptor alpha 1 (IL13RA1) (Interleukin 13 Receptor, alpha 1 (IL13RA1))

Alternative Name: IL-13Ra1

Background: Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Molecular Weight: 39.5 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result.

UniProt: P78552-1

Pathways: JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response