ITGAV/ITGB5 Protein (AA 31-992, Heterodimer) (Biotin,His-Avi Tag)

Catalog No. ABIN7275062

Recombinant ITGAV/ITGB5 protein expressed in HEK-293 Cells.

Quick Overview for ITGAV/ITGB5 Protein (AA 31-992, Heterodimer) (Biotin,His-Avi Tag) (ABIN7275062)

Target: ITGAV/ITGB5 (Integrin alpha V, Integrin beta 5 (ITGAV/ITGB5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 31-992, Heterodimer

Purification tag / Conjugate: This ITGAV/ITGB5 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein

Sequence: Phe31-Val992(ITGAV) acidic tail & Gly24-Asn719(ITGB5) basic tail

Specificity: Uni-Prot: P06756-1 (ITGAV), P18084 (ITGB5)

Characteristics: Recombinant Biotinylated Human Integrin alpha V beta 5 (ITGAV&ITGB5) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Gly24-Asn719(ITGB5) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human ITGAV&ITGB5, His Tag at 1ug/ml (100ul/Well) on streptavidin (5ug/ml) precoated plate. Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).,Biotinylated Human ITGAV&ITGB5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB5 (Integrin alpha V, Integrin beta 5 (ITGAV/ITGB5))

Alternative Name: ITGAV&ITGB5

Background: Integrin ITGAV & ITGB5 is a type of integrin that binds to matrix macromolecules and proteinases and thereby stimulates angiogenesis. However, it inhibits angiogenesis. It consists of two components, integrin alpha V and integrin beta 5. Integrin ITGAV & ITGB5 is a receptor for fibronectin. It recognizes the sequence R-G-D in its ligand.(Microbial infection)  Integrin ITGAV & ITGB5 acts as a receptor for adenovirus type C.

Molecular Weight: 114 kDa (ITGAV) & 81.4 kDa (ITGB5). Due to glycosylation, the protein migrates to 85-140 kDa based on Bis-Tris PAGE result.

UniProt: P06756