ITGAV/ITGB8 Protein (AA 31-992, Heterodimer) (Biotin,His-Avi Tag)

Catalog No. ABIN7275066

This Recombinant ITGAV/ITGB8 protein is produced in HEK-293 Cells.

Quick Overview for ITGAV/ITGB8 Protein (AA 31-992, Heterodimer) (Biotin,His-Avi Tag) (ABIN7275066)

Target: ITGAV/ITGB8 (Integrin alpha V, Integrin beta 8 (ITGAV/ITGB8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-992, Heterodimer

Purification tag / Conjugate: This ITGAV/ITGB8 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein

Sequence: Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail

Specificity: Uni-Prot: P06756-1 (ITGAV), P26012-1 (ITGB8)

Characteristics: Recombinant Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Glu43-Arg684(ITGB8) basic tail.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.,Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA.,Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ITGAV/ITGB8 (Integrin alpha V, Integrin beta 8 (ITGAV/ITGB8))

Alternative Name: ITGAV&ITGB8

Background: Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.

Molecular Weight: 114 kDa (ITGAV) and 76 kDa (ITGB8). Due to glycosylation, the protein migrates to 85-145 kDa based on Bis-Tris PAGE result.

UniProt: P06756