LCAT Protein (AA 25-438) (His tag)

Catalog No. ABIN7275131

Product Details

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Protein Type: Recombinant

Protein Characteristics: AA 25-438

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This LCAT protein is labelled with His tag.

Purpose: Mouse LCAT Protein

Sequence: Phe25-Glu438

Characteristics: Recombinant Mouse LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu438.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))

Alternative Name: LCAT (LCAT Products)

Background: Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.

Molecular Weight: 48.3 kDa. Due to glycosylation, the protein migrates to 67-75 kDa based on Tris-Bis PAGE result.

UniProt: P16301

Pathways: Lipid Metabolism