LCAT Protein (AA 25-440) (His tag)
-
- Target See all LCAT Proteins
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-440
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LCAT protein is labelled with His tag.
- Purpose
- Human LCAT Protein
- Sequence
- Phe25-Glu440
- Characteristics
- Recombinant Human LCAT Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe25-Glu440.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human LCAT, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LCAT Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail.
-
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)
LCAT Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 1-440) protein (GST tag)LCAT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 98-197) protein (GST tag)LCAT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-440) protein (His tag)Crystallography grade LCAT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Lecithin-Cholesterol Acyltransferase (LCAT) (AA 25-438) protein (His tag)Crystallography grade LCAT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- LCAT (Lecithin-Cholesterol Acyltransferase (LCAT))
- Alternative Name
- LCAT (LCAT Products)
- Synonyms
- AI046659 Protein, D8Wsu61e Protein, MGC82035 Protein, lcat Protein, MGC88964 Protein, LCAT Protein, lecithin-cholesterol acyltransferase Protein, lecithin cholesterol acyltransferase Protein, lecithin-cholesterol acyltransferase L homeolog Protein, solute carrier family 12 member 4 Protein, fragile site, aphidicolin type, common, fra(13)(q13.2) Protein, LCAT Protein, Lcat Protein, lcat.L Protein, lcat Protein, SLC12A4 Protein, FRA13A Protein
- Background
- Lecithin:cholesterol acyltransferase (LCAT) is the only enzyme capable of esterifying cholesterol in plasma, thus determining the maturation of high-density lipoproteins. Because it maintains an unesterified cholesterol gradient between peripheral cells and extracellular acceptors, for a long time, LCAT has been considered as a key enzyme in reverse cholesterol transport.
- Molecular Weight
- 48.2 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
- UniProt
- P04180
- Pathways
- Lipid Metabolism
-
