SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, C.37 - Lambda, RBD) (His tag)

Catalog No. ABIN7275136

This Recombinant SARS-CoV-2 Spike protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV-2 Spike Protein (SARS-CoV-2 S) (AA 319-541, C.37 - Lambda, RBD) (His tag) (ABIN7275136)

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus-2 (SARS-CoV-2)

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 319-541, C.37 - Lambda, RBD

Purification tag / Conjugate: This SARS-CoV-2 Spike protein is labelled with His tag.

Purpose: SARS-CoV-2 Spike RBD (Lambda C.37) Protein

Sequence: Arg319-Phe541(L452Q, F490S)

Characteristics: Recombinant SARS-CoV-2 Spike RBD (Lambda C.37) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(L452Q, F490S).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized SARS-CoV-2 Spike RBD (Lambda C.37) , His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.,SARS-CoV-2 Spike RBD (Lambda C.37) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-CoV-2 Spike RBD (Lambda C.37) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SARS-CoV-2 Spike (SARS-CoV-2 S)

Alternative Name: SARS-CoV-2 Spike

Target Type: Viral Protein

Background: The ongoing coronavirus disease 2019 (COVID-19) pandemic has prioritized the development of small-animal models for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The resulting mouse-adapted strain at passage 6 (called MASCp6) showed increased infectivity in mouse lung and led to interstitial pneumonia and inflammatory responses in both young and aged mice after intranasal inoculation. Deep sequencing revealed a panel of adaptive mutations potentially associated with the increased virulence. In particular, the N501Y mutation is located at the receptor binding domain (RBD) of the spike protein.

Molecular Weight: 26.15 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.