LGR5 Protein (AA 22-543) (hFc Tag)

Catalog No. ABIN7275146

Recombinant LGR5 protein expressed in HEK-293 Cells.

Quick Overview for LGR5 Protein (AA 22-543) (hFc Tag) (ABIN7275146)

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 22-543

Purification tag / Conjugate: This LGR5 protein is labelled with hFc Tag.

Purpose: Human LGR-5 Protein

Sequence: Gly22-Pro543

Characteristics: Recombinant Human LGR-5 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly22-Pro543.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LGR-5, hFc Tag captured on CM5 Chip via Protein A can bind Human R-Spondin 3, His Tag with an affinity constant of 0.59 nM as determined in SPR assay (Biacore T200).,Human LGR-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 5 mM DTT ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LGR5 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5 (LGR5))

Alternative Name: LGR-5

Background: G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.LGR5 is a new functional GSC marker and prognostic indicator that can promote EMT by activating the Wnt/β-catenin pathway and would thus be a novel therapeutic target for glioma.

Molecular Weight: 85 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.

UniProt: O75473

Pathways: WNT Signaling