LIF Protein (AA 23-202)

Catalog No. ABIN7275151

This Recombinant LIF protein is expressed in HEK-293 Cells.

Quick Overview for LIF Protein (AA 23-202) (ABIN7275151)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-202

Purpose: Human LIF Protein

Sequence: Ser23-Phe202

Characteristics: Recombinant Human LIF Protein is expressed from HEK293 without tag.It contains Ser23-Phe202.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, hFc Tag with the EC50 of 0.10ug/ml determined by ELISA (QC Test).,Human LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Determined by its dose-dependent ability to induce reporter gene in 293T-STAT3-Luc2 reporter cells. The ED50-for this effect is 0.2-2 ng/mL.,The purity of Human LIF is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Molecular Weight: 19.7 kDa. Due to glycosylation, the protein migrates to 40-55 kDa based on Bis-Tris PAGE result.

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding