LIF Protein (AA 25-203) (hFc Tag)

Catalog No. ABIN7275154

This Recombinant LIF protein is expressed in HEK-293 Cells.

Quick Overview for LIF Protein (AA 25-203) (hFc Tag) (ABIN7275154)

Target: LIF (Leukemia Inhibitory Factor (LIF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 25-203

Purification tag / Conjugate: This LIF protein is labelled with hFc Tag.

Purpose: Mouse LIF Protein

Sequence: Pro25-Phe203

Characteristics: Recombinant Mouse LIF Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Pro25-Phe203.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse LIF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse LIF is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF

Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy.

Molecular Weight: 46.5 kDa. Due to glycosylation, the protein migrates to 67-70 kDa based on Bis-Tris PAGE result.

UniProt: P09056

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding