LILRB2 Protein (AA 22-229) (His tag)

Catalog No. ABIN7275165

This Recombinant LILRB2 protein is produced in HEK-293 Cells.

Quick Overview for LILRB2 Protein (AA 22-229) (His tag) (ABIN7275165)

Target: LILRB2 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Product Details

Protein Characteristics: AA 22-229

Purification tag / Conjugate: This LILRB2 protein is labelled with His tag.

Purpose: Human LILRB2/CD85d/ILT4 Domain1&2 Protein

Sequence: Gln22-Val229

Characteristics: Recombinant Human LILRB2/CD85d/ILT4 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln22-Val229.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human HLA-G Complex Tetramer, His Tag immobilized on CM5 Chip can bind Human LILRB2 Domain1&2, His Tag with an affinity constant of 6.5uM as determined in a SPR assay (Biacore T200).,Immobilized Human LILRB2 Domain1&2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 3.1ng/ml determined by ELISA (QC Test).,Human LILRB2 Domain 1&2 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.,Serial dilutions of Anti-LILRB2 Antibody were added into Human LILRB2 Domain1&2, His Tag : Biotinylated Human HLA-G Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.43ug/ml.,The purity of-Human LILRB2 Domain 1&2 is greater than 90% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LILRB2 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))

Alternative Name: LILRB2

Background: The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.

Molecular Weight: 24.2 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.

Pathways: Cellular Response to Molecule of Bacterial Origin