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LILRB2 Protein (AA 22-457) (mFc Tag)

This Recombinant LILRB2 protein is expressed in HEK-293 Cells.

SDS

Bind

SEC-HPLC

Bind

Immobilized Human LILRB2, mFc Tag at 0. (LILRB2 Protein (AA 22-457) (mFc Tag))

ELISA

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Catalog No. ABIN7275172

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Quick Overview for LILRB2 Protein (AA 22-457) (mFc Tag) (ABIN7275172)

Target

See all LILRB2 Proteins

LILRB2 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))

Protein Type

Recombinant

Biological Activity

Active

Origin

Human

Source

HEK-293 Cells

Purity

> 95 % as determined by Bis-Tris PAGE

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Protein Characteristics

AA 22-457

Purification tag / Conjugate

This LILRB2 protein is labelled with mFc Tag.

Purpose

Human LILRB2/CD85d/ILT4 Protein

Sequence

Gln22-Arg457

Characteristics

Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Gln22-Arg457.

Sterility

0.22 μm filtered

Endotoxin Level

Less than 1 EU per μg by the LAL method.

Biological Activity Comment

Immobilized Human LILRB2, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA (QC Test).,Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-LILRB2 Antibody were added into Human HLA-G Complex Tetramer, His Tag : Human LILRB2, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.11ug/ml.
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Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-229) (Active) protein (His tag)
LILRB2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-457) (Active) protein (His-Avi Tag)
LILRB2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-457) (Active) protein (Biotin,His-Avi Tag)
LILRB2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 24-457) (Active) protein (His tag)
LILRB2 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 24-457) (Active) protein (hFc Tag)
LILRB2 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-461) protein (His tag)
LILRB2 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-598) protein (GST tag)
LILRB2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-458) protein (Fc Tag)
LILRB2 Origin: Human Host: Mammalian Cells Recombinant Greater than 95 % as determined by SDS-PAGE. WB, ELISA

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 22-458) protein (His tag)
LILRB2 Origin: Human Host: Mammalian Cells Recombinant Greater than 95 % as determined by SDS-PAGE. WB, ELISA

Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2) (AA 1-461) protein (His tag)
LILRB2 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE.

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer

Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage

-20 °C,-80 °C

Storage Comment

-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date

12 months
Target

LILRB2 (Leukocyte Immunoglobulin-Like Receptor, Subfamily B (With TM and ITIM Domains), Member 2 (LILRB2))

Alternative Name

LILRB2

Background

The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.

Molecular Weight

73.6 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.

Pathways

Cellular Response to Molecule of Bacterial Origin