LILRA3 Protein (AA 24-439) (His-Avi Tag)

Catalog No. ABIN7275177

This Recombinant LILRA3 protein is produced in HEK-293 Cells.

Quick Overview for LILRA3 Protein (AA 24-439) (His-Avi Tag) (ABIN7275177)

Target: LILRA3 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (Without TM Domain), Member 3 (LILRA3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-439

Purification tag / Conjugate: This LILRA3 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human LILRA3/CD85e Protein

Sequence: Gly24-Glu439

Characteristics: Recombinant Human LILRA3/CD85e Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu439.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LILRA3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human LILRA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4).Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LILRA3 (Leukocyte Immunoglobulin-Like Receptor, Subfamily A (Without TM Domain), Member 3 (LILRA3))

Alternative Name: LILRA3

Background: Human LILRA3, also known as Leukocyte Ig-like receptor 4 (LIR-4), CD85 antigen-like family member E, Immunogobulin-like transcript 6 (ILT-6) and monocyte inhibitory receptor HM43/HM31,  is a 70 kD Ig superfamily member that belongs to the leukocyte receptor complex/cluster. Mature LILRA3 is 416 amino acids (aa) in length and contains four C2-type Ig-like domains. Unlike other LILR family members, LILRA3 is actively secreted.LILRA3 acts as soluble receptor for class I MHC antigens. LILRA3 binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

Molecular Weight: 47.9 kDa. Due to glycosylation, the protein migrates to 68-72 kDa based on Bis-Tris PAGE result.

UniProt: Q8N6C8