LRG1 Protein (AA 36-347) (His tag)

Catalog No. ABIN7275202

This Recombinant LRG1 protein is expressed in HEK-293 Cells.

Quick Overview for LRG1 Protein (AA 36-347) (His tag) (ABIN7275202)

Target: LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 36-347

Purification tag / Conjugate: This LRG1 protein is labelled with His tag.

Purpose: Human LRG1 Protein

Sequence: Val36-Gln347

Characteristics: Recombinant Human LRG1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val36-Gln347.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human LRG1, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRG1 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA(QC Test).,Human LRG1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human LRG1 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: LRG1 (Leucine-Rich alpha-2 Glycoprotein 1 (LRG1))

Alternative Name: LRG1

Background: Diabetic nephropathy (DN) is an important public health concern of increasing proportions and the leading cause of end-stage renal disease (ESRD) in diabetic patients. It is one of the most common long-term microvascular complications of diabetes mellitus that is characterized by proteinuria and glomerular structural changes. LRG1 is a novel pro-angiogenic factors involved in the abnormal angiogenesis and renal fibrosis in DN.

Molecular Weight: 35.4 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result.

UniProt: P02750

Pathways: Brown Fat Cell Differentiation