LRP5 Protein (AA 644-1263) (hFc Tag)

Catalog No. ABIN7275208

This Recombinant LRP5 protein is expressed in HEK-293 Cells.

Quick Overview for LRP5 Protein (AA 644-1263) (hFc Tag) (ABIN7275208)

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 644-1263

Purification tag / Conjugate: This LRP5 protein is labelled with hFc Tag.

Purpose: Human LRP-5 Protein

Sequence: Glu644-Gln1263

Characteristics: Recombinant Human LRP-5 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu644-Gln1263.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human LRP-5, hFc Tag captured on CM5 Chip via Protein A can bind Human DKK1, His Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).,Immobilized Biotinylated Human DKK1, His Avi Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human LRP-5, hFc Tag with the EC50 of 61.3ng/ml determined by ELISA (QC Test).,Human LRP-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS ( pH 7.4).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRP5 (Low Density Lipoprotein Receptor-Related Protein 5 (LRP5))

Alternative Name: LRP-5

Background: A role for low-density lipoprotein-related receptor 5 (LRP5) in human bone was first established by the identification of genetic alterations that led to dramatic changes in bone mass. Shortly thereafter, mutations that altered the function of the sclerostin (SOST) gene were also associated with altered human bone mass. Subsequent studies of LRP5 and sclerostin have provided important insights into the mechanisms by which these proteins regulate skeletal homeostasis.

Molecular Weight: 96.51 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Bis-Tris PAGE result.

UniProt: O75197

Pathways: WNT Signaling, Stem Cell Maintenance, Positive Regulation of fat Cell Differentiation