LRP6 Protein (AA 20-630) (mFc Tag)

Catalog No. ABIN7275210

This Recombinant LRP6 protein is produced in HEK-293 Cells.

Quick Overview for LRP6 Protein (AA 20-630) (mFc Tag) (ABIN7275210)

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Tris-Bis PAGE

Product Details

Protein Characteristics: AA 20-630

Purification tag / Conjugate: This LRP6 protein is labelled with mFc Tag.

Purpose: Human LRP-6 Protein

Sequence: Ala20-Pro630

Characteristics: Recombinant Human LRP-6 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala20-Pro630.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human DKK1, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 2.1μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))

Alternative Name: LRP-6

Background: Dickkopf-related protein 1 (DKK-1), a ligand for the WNT coreceptors low-density lipoprotein receptor-related proteins 5 and 6 (LRP-5 and LRP-6) and an inhibitor of WNT/β-catenin signaling, effectively inhibits pericyte activation, detachment, and transition to myofibroblasts in vivo in response to kidney injury, resulting in attenuated fibrogenesis, capillary rarefaction, and inflammation.LRP-6 interacts closely with PDGF receptor β and TGF-β receptor 1 at the cell membrane, suggesting that it may have roles in pathways other than WNT/β-catenin.

Molecular Weight: 95.3 kDa. Due to glycosylation, the protein migrates to 110-115 kDa based on Tris-Bis PAGE result.

UniProt: O75581-1

Pathways: WNT Signaling, Tube Formation