LRP6 Protein (AA 20-630) (His-Avi Tag,Biotin)
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- Target See all LRP6 Proteins
- LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 20-630
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LRP6 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human LRP-6 (20-630) Protein, His,Avitag™
- Sequence
- Ala 20 - Pro 630
- Characteristics
- Biotinylated Human LRP-6, His,Avitag (LR6-H82E7) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Pro 630 (Accession # O75581-1 ).
- Purity
- >90 % as determined by SDS-PAGE.
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product LRP6 Protein
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Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (AA 20-630) protein (mFc Tag)
LRP6 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE
Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) (AA 20-630) (Active) protein (Fc Tag)LRP6 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- Application Notes
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 72.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition due to glycosylation.
- Comment
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- LRP6 (Low Density Lipoprotein Receptor-Related Protein 6 (LRP6))
- Alternative Name
- LRP-6 (LRP6 Products)
- Synonyms
- lrp6-A Protein, Xlrp6 Protein, adcad2 Protein, lrp6 Protein, LRP6 Protein, ADCAD2 Protein, C030016K15Rik Protein, Cd Protein, Gw Protein, LDL receptor related protein 6 Protein, LDL receptor related protein 6 L homeolog Protein, LDL receptor related protein 6 S homeolog Protein, low density lipoprotein receptor-related protein 6 Protein, low-density lipoprotein receptor-related protein 6 Protein, LRP6 Protein, lrp6.L Protein, lrp6.S Protein, lrp6 Protein, Lrp6 Protein, LOC590395 Protein
- Background
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Synonyms: LRP-6,LRP6,
Description: LRP6 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP5. Low-density lipoprotein receptor-related protein 6 is a key component of the LRP5/LRP6/Frizzled co-receptor group that is involved in canonical Wnt pathway. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. - Molecular Weight
- 72.5 kDa
- NCBI Accession
- NP_002327
- Pathways
- WNT Signaling, Tube Formation
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