MICA Protein (AA 24-308) (His-Avi Tag,Biotin)

Catalog No. ABIN7275256

This Recombinant MICA protein is expressed in HEK-293 Cells.

Quick Overview for MICA Protein (AA 24-308) (His-Avi Tag,Biotin) (ABIN7275256)

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 24-308

Purification tag / Conjugate: This MICA protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human MICA Protein

Sequence: Glu24-Gln308

Characteristics: Recombinant Biotinylated Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Gln308.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA

Background: TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.

Molecular Weight: 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.

UniProt: Q96QC4

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis, Human Leukocyte Antigen (HLA) in Adaptive Immune Response