MICA Protein (His tag)

Catalog No. ABIN7319284

Product Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This MICA protein is labelled with His tag.

Purpose: Recombinant Human MICA Protein (His Tag)(Active)

Sequence: Glu24-Gln308

Characteristics: Recombinant Human MHC Class I Polypeptide-Related Sequence A is produced by our Mammalian expression system and the target gene encoding Glu24-Gln308 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Human NKG2DL-Fc(Cat: PKSH032815) at 10μg/ml(100 μl/well) can bind Human MICA-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA (MICA Products)

Synonyms: MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein

Background:

Background: MHC Class I Polypeptide-Related Sequence A (MICA) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. Unlike classical MHC class I molecules, MICA does not form a heterodimer with beta-2-microglobulin. MICA shares 85 % amino acid identity with a closely related protein, MICB. MICA acts as a stress-induced self-antigen that is recognized by NK cells, NKT cells, and most of the subtypes of T cells. As a Ligand for the KLRK1/NKG2D receptor, MICA binds to KLRK1 leads to cell lysis. MICA functions as an antigen for gamma delta T cells and is frequently expressed in epithelial tumors. MICA antigens are able to elicit the synthesis of alloantibodies in transplant recipients. Studies have shown that anti-MICA antibodies are associated with acute renal allograft rejection and failure. MICA recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.

Synonym: MHC Class I Polypeptide-Related Sequence A, MIC-A, MICA, PERB11.1

Molecular Weight: 33.9 kDa

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis