MME Protein (AA 52-750) (His tag)

Catalog No. ABIN7275263

Recombinant MME protein expressed in HEK-293 Cells.

Quick Overview for MME Protein (AA 52-750) (His tag) (ABIN7275263)

Target: MME (Membrane Metallo-Endopeptidase (MME))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 52-750

Purification tag / Conjugate: This MME protein is labelled with His tag.

Purpose: Human CD10/MME Protein

Sequence: Tyr52-Trp750

Characteristics: Recombinant Human CD10/MME Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr52-Trp750.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human CD10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH. The specific activity is > 1500 pmoles/min/ug.,The purity of Human CD10 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 50 mM MES, 100 mM NaCl, 1 mM ZnCl2, 10 % Glycerol ( pH 6.5).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MME (Membrane Metallo-Endopeptidase (MME))

Alternative Name: CD10

Background: CD10 is an endopeptidase that degrades various bioactive peptides in the extracellular matrix. In addition to enzymatic degradation, it affects multiple intracellular signal transduction pathways. CD10 expression has been extensively studied in human epithelial cancers of numerous organs and sites. CD10 expression pattern depended on the histotypes of thyroid lesions. When possible metastatic tumours and non-epithelial tumours are excluded, high CD10 expression may be useful in determining whether a primary thyroid carcinoma includes an anaplastic component.

Molecular Weight: 80.9 kDa. Due to glycosylation, the protein migrates to 81-110 kDa based on Bis-Tris PAGE result.

UniProt: P08473

Pathways: RTK Signaling, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration