MMP8 Protein (AA 21-467) (His tag)

Catalog No. ABIN7275265

This Recombinant MMP8 protein is expressed in HEK-293 Cells.

Quick Overview for MMP8 Protein (AA 21-467) (His tag) (ABIN7275265)

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-467

Purification tag / Conjugate: This MMP8 protein is labelled with His tag.

Purpose: Cynomolgus MMP-8 Protein (pro form)

Sequence: Phe21-Ser467

Characteristics: Recombinant Cynomolgus MMP-8 Protein is expressed from HEK293 with His tag at the C-Terminus. The protein needs to be activated by APMA to have hydrolytic activity.It contains Phe21-Ser467.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus MMP-8, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MMP-8 Antibody, hFc Tag with the EC50 of 20.2ng/ml determined by ELISA (QC tested).,Cynomolgus MMP-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is >200 pmol/min/ug (QC tested).,The purity of Cynomolgus MMP-8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 50 mM Tris, 150 mM NaCL,10 mM CaCI2 ( pH 7.4).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: MMP8 (Matrix Metallopeptidase 8 (Neutrophil Collagenase) (MMP8))

Alternative Name: MMP-8

Background: Alteration of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) expression has been studied for various cardiac diseases, including dilated cardiomyopathy (DCM), with the significance of surrogate markers of extracellular matrix (ECM) remodeling. MMP-8 was identified only in myocardiocytes, while MMP-9 and TIMP-2 were present in both myocardiocytes and stroma, but with different intensity. The increasing intensity of MMP-8 and TIMP-2 immunoreactions was significantly associated with low HCS.

Molecular Weight: 52.27 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005579523