PVRL4 Protein (AA 32-351) (His tag)

Catalog No. ABIN7275304

This Recombinant PVRL4 protein is expressed in HEK-293 Cells.

Quick Overview for PVRL4 Protein (AA 32-351) (His tag) (ABIN7275304)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-351

Purification tag / Conjugate: This PVRL4 protein is labelled with His tag.

Purpose: Cynomolgus Nectin-4 Protein

Sequence: Gly32-Val351

Characteristics: Recombinant Cynomolgus Nectin-4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly32-Val351.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus Nectin-4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.,Cynomolgus Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of Cynomolgus Nectin-4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: Nectin-4

Background: NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life.

Molecular Weight: 35.9 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005541277

Pathways: Cell-Cell Junction Organization