PVRL4 Protein (AA 32-351) (His tag)

Catalog No. ABIN7275304

This Recombinant PVRL4 protein is expressed in HEK-293 Cells.

Quick Overview for PVRL4 Protein (AA 32-351) (His tag) (ABIN7275304)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Protein Type: Recombinant

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-351

Purification tag / Conjugate: This PVRL4 protein is labelled with His tag.

Purpose: Cynomolgus Nectin-4 Protein

Sequence: Gly32-Val351

Characteristics: Recombinant Cynomolgus Nectin-4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly32-Val351.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus Nectin-4, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: Nectin-4

Background: Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

Molecular Weight: 35.9 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Tris-Bis PAGE result.

NCBI Accession: XP_005541277

Pathways: Cell-Cell Junction Organization