PVRL4 Protein (AA 32-349) (hFc Tag)

Catalog No. ABIN7275306

This Recombinant PVRL4 protein is produced in HEK-293 Cells.

Quick Overview for PVRL4 Protein (AA 32-349) (hFc Tag) (ABIN7275306)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 32-349

Purification tag / Conjugate: This PVRL4 protein is labelled with hFc Tag.

Purpose: Human Nectin-4 Protein

Sequence: Gly32-Ser349

Characteristics: Recombinant Human Nectin-4 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gly32-Ser349.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).,Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test).,Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.,The purity of-Human Nectin-4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: Nectin-4

Background: NECTIN-4 [a poliovirus receptor-related-4 (pvrl-4) encoded protein] is a Ca2+ independent immunoglobulin-like protein. Along with other Nectins (Nectin-1, -2 and -3), it is primarily involved in cell-cell adhesion. In contrast to other Nectins, Nectin-4 is specifically enriched in the embryonic and placental tissues but its expression significantly declines in adult life.

Molecular Weight: 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Bis-Tris PAGE result.

UniProt: Q96NY8

Pathways: Cell-Cell Junction Organization