NOG Protein (AA 28-232)

Catalog No. ABIN7275358

This Recombinant NOG protein is expressed in CHO Cells.

Quick Overview for NOG Protein (AA 28-232) (ABIN7275358)

Target: NOG (Noggin (NOG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-232

Purpose: Human Noggin Protein

Sequence: Gln28-Cys232

Characteristics: Recombinant Human Noggin Protein is expressed from CHO without tag.It contains Gln28-Cys232.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.05 EU per μg by the LAL method.

Biological Activity Comment: Human Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is-4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP-4.,The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: NOG (Noggin (NOG))

Alternative Name: Noggin

Background: Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β-catenin, EGFR, ERK and Akt.

Molecular Weight: 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Bis-Tris PAGE result.

UniProt: Q13253

Pathways: Stem Cell Maintenance, Tube Formation