Oncostatin M Receptor Protein (OSMR) (AA 28-737) (His tag)

Catalog No. ABIN7275383

Recombinant Oncostatin M Receptor protein expressed in HEK-293 Cells.

Quick Overview for Oncostatin M Receptor Protein (OSMR) (AA 28-737) (His tag) (ABIN7275383)

Target: Oncostatin M Receptor (OSMR)

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 28-737

Purification tag / Conjugate: This Oncostatin M Receptor protein is labelled with His tag.

Purpose: Cynomolgus OSMR beta Protein

Sequence: Glu28-Ser737

Characteristics: Recombinant Cynomolgus OSMR beta Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu28-Ser737.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus OSMR beta, His Tag at 2 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 8.4 ng/ml determined by ELISA.,Cynomolgus OSMR beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Anti-OSMR Antibody, hFc Tag on ProA-Biosensor can bind Cynomolgus OSMR beta, His Tag with an affinity constant of 1.75 nM as determined in BLI assay (Gator® Prime).,The purity of Cynomolgus OSMR beta is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Oncostatin M Receptor (OSMR)

Alternative Name: OSMR

Background: OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.

Molecular Weight: 82.04 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5UFW5

Pathways: JAK-STAT Signaling, Growth Factor Binding