PLAU Protein (AA 21-430) (His tag)

Catalog No. ABIN7275453

This Recombinant PLAU protein is produced in HEK-293 Cells.

Quick Overview for PLAU Protein (AA 21-430) (His tag) (ABIN7275453)

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-430

Purification tag / Conjugate: This PLAU protein is labelled with His tag.

Purpose: Cynomolgus PLAU/uPA Protein (active form)

Sequence: Ser21-Leu430

Characteristics: Recombinant Cynomolgus PLAU/uPA Protein (active form) is expressed from HEK293 with His tag at the C-Terminus.It contains Ser21-Leu430, which consists of two chains: Long chain A and chain B. The long chain A is further cleaved to yield a short chain A and N-Terminus fragment.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Cynomolgus uPAR, His Tag immobilized on CM5 Chip can bind Cynomolgus PLAU, His Tag with an affinity constant of 0.033 nM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus PLAU, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human uPAR, hFc Tag with the EC50 of 21.4ng/ml determined by ELISA (QC Test).,Cynomolgus PLAU (active form) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2000 pmol/min/ug (QC Test).,The purity of Cynomolgus PLAU (active form) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: PLAU

Background: Plasminogen activator, urokinase (uPA) is a secreted serine protease whose Dysregulation is often accompanied by various cancers. PLAU inhibition could suppress tumor growth. Collectively, PLAU is necessary for tumor progression and can be a diagnostic and prognostic biomarker in HNSCC.

Molecular Weight: 47.23 kDa. Due to protein lysis and glycosylation, the protein migrates to 23-25 kDa, 35-40 kDa and 50-60 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5WND1

Pathways: Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration