PLA2G1B Protein (AA 23-148) (hFc Tag)

Catalog No. ABIN7275459

This Recombinant PLA2G1B protein is produced in HEK-293 Cells.

Quick Overview for PLA2G1B Protein (AA 23-148) (hFc Tag) (ABIN7275459)

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-148

Purification tag / Conjugate: This PLA2G1B protein is labelled with hFc Tag.

Purpose: Human PLA2G1B Protein

Sequence: Ala23-Ser148

Characteristics: Recombinant Human PLA2G1B Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala23-Ser148.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human PLA2G1B on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human PLA2G1B is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: PLA2G1B (Phospholipase A2, Group IB (PLA2G1B))

Alternative Name: PLA2G1B

Background: Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction. PLA2 provides precursors for generation of eicosanoids, such as prostaglandins (PGs) and leukotrienes (LTs), when the cleaved fatty acid is arachidonic acid, platelet-activating factor (PAF) when the sn-1 position of the phosphatidylcholine contains an alkyl ether linkage and some bioactive lysophospholipids, such as lysophosphatidic acid (lysoPA).

Molecular Weight: 40.9 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Bis-Tris PAGE result.

UniProt: P04054

Pathways: Inositol Metabolic Process, VEGF Signaling