PRNP Protein (AA 23-230) (hFc Tag)
Quick Overview for PRNP Protein (AA 23-230) (hFc Tag) (ABIN7275480)
Target
See all PRNP ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Protein Characteristics
- AA 23-230
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Purification tag / Conjugate
- This PRNP protein is labelled with hFc Tag.
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Purpose
- Human PRNP Protein
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Sequence
- Lys23-Ser230
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Characteristics
- Recombinant Human PRNP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Lys23-Ser230.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human PRNP, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 41.7ng/ml determined by ELISA.,Human PRNP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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Prion Protein (PRNP) (AA 1-73) protein (Fc Tag)
custom-made PRNP Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Prion Protein (PRNP) (AA 23-230) (Active) protein (hFc Tag)PRNP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Prion Protein (PRNP) (AA 90-230) protein (Fc Tag)PRNP Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Prion Protein (PRNP) (AA 1-253) protein (GST tag)PRNP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Prion Protein (PRNP) (AA 23-229) protein (Fc Tag)PRNP Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Prion Protein (PRNP) (AA 29-231) protein (His tag)PRNP Origin: Rat Host: Yeast Recombinant > 90 % SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- PRNP (Prion Protein (PRNP))
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Alternative Name
- PRNP
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Background
- Prion protein gene (PRNP) variants determine the susceptibility of humans, sheep and mice to prion diseases, whereas polymorphisms in the open reading frame (ORF) of bovine PRNP seem to be unrelated to the incidence of bovine spongiform encephalopathy (BSE). According to the latest reports, the genetic susceptibility of cattle to BSE is associated with polymorphisms ofthe regulatory region of the PRNP gene and the level ofits expression.
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Molecular Weight
- 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Bis-Tris PAGE result.
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UniProt
- P04156
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Pathways
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
Target
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