PRNP Protein (AA 23-230) (Fc Tag)
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- Target See all PRNP Proteins
- PRNP (Prion Protein (PRNP))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 23-230
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRNP protein is labelled with Fc Tag.
- Purpose
- Human PRNP Protein
- Sequence
- Lys23-Ser230
- Characteristics
- Recombinant Human PRNP Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys23-Ser230.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human PRNP, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PRNP Antibody, hFc Tag with the EC50 of 25.8ng/ml determined by ELISA. See testing image for detail.
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Prion Protein (PRNP) (AA 23-230) protein (Fc Tag)
PRNP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Prion Protein (PRNP) (AA 1-253) protein (GST tag)PRNP Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Prion Protein (PRNP) (AA 1-73) protein (Strep Tag)Crystallography grade PRNP Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Prion Protein (PRNP) (AA 29-231) protein (His tag)PRNP Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Prion Protein (PRNP) proteinPRNP Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % ELISA, SDS, WB, IP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- PRNP (Prion Protein (PRNP))
- Alternative Name
- PRNP (PRNP Products)
- Synonyms
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ASCR Protein, AltPrP Protein, CD230 Protein, CJD Protein, GSS Protein, KURU Protein, PRIP Protein, PrP Protein, PrP27-30 Protein, PrP33-35C Protein, PrPc Protein, p27-30 Protein, Prn Protein, Prn-p Protein, prp Protein, cjd Protein, prion Protein, prp(c) Protein, MGC84114 Protein, PRP Protein, PRPC Protein, Prp Protein, SIP Protein, PRNP Protein, AA960666 Protein, AI325101 Protein, PrP
Protein, PrPC Protein, PrPSc Protein, Prn-i Protein, Sinc Protein, prP27-30 Protein, prP33-35C Protein, PrP-1 Protein, PrP1 Protein, prnprs1 Protein, stPrP-1 Protein, zgc:123004 Protein, prion protein Protein, prion protein S homeolog Protein, prion protein b Protein, PRNP Protein, Prnp Protein, prnp.S Protein, prnpb Protein - Background
- Prion protein gene (PRNP) variants determine the susceptibility of humans, sheep and mice to prion diseases, whereas polymorphisms in the open reading frame (ORF) of bovine PRNP seem to be unrelated to the incidence of bovine spongiform encephalopathy (BSE). According to the latest reports, the genetic susceptibility of cattle to BSE is associated with polymorphisms ofthe regulatory region of the PRNP gene and the level ofits expression.
- Molecular Weight
- 49.4 kDa. Due to glycosylation, the protein migrates to 60-67 kDa based on Tris-Bis PAGE result.
- UniProt
- P04156
- Pathways
- Transition Metal Ion Homeostasis, Activated T Cell Proliferation
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