RAGE Protein (AA 23-340) (His tag)

Catalog No. ABIN7275509

Recombinant RAGE protein expressed in HEK-293 Cells.

Quick Overview for RAGE Protein (AA 23-340) (His tag) (ABIN7275509)

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 23-340

Purification tag / Conjugate: This RAGE protein is labelled with His tag.

Purpose: Mouse AGER Protein

Sequence: Gly23-Ala340

Characteristics: Recombinant Mouse AGER Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly23-Ala340.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse AGER on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse AGER is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Alternative Name: RAGE

Background: The receptor for advanced glycation end-products (RAGE) is a cell surface transmembrane multiligand receptor, encoded by the AGER gene. RAGE presents many transcripts, is expressed mainly in the lung, and involves multiple pathways (such as NFκB, Akt, p38, and MAP kinases) that initiate and perpetuate an unfavorable proinflammatory state.

Molecular Weight: 34.85 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_031451

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins