ROBO4 Protein (AA 27-469) (His tag)

Catalog No. ABIN7275523

This Recombinant ROBO4 protein is expressed in HEK-293 Cells.

Quick Overview for ROBO4 Protein (AA 27-469) (His tag) (ABIN7275523)

Target: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 27-469

Purification tag / Conjugate: This ROBO4 protein is labelled with His tag.

Purpose: Human ROBO4 Protein

Sequence: Ala27-Glu469

Characteristics: Recombinant Human ROBO4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala27-Glu469.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ROBO4, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-ROBO4 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).,Human ROBO4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ROBO4 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Alternative Name: ROBO4

Background: Roundabout4 (Robo4) is a transmembrane receptor that belongs to the Roundabout (Robo) family of axon guidance molecules. Robo4 is an endothelial-specific receptor that participates in endothelial cell migration, proliferation, and angiogenesis and the maintenance of vasculature homeostasis. Robo4 is a promising and potentially valuable therapeutic target for treating pathological angiogenesis and developmental defects in angiogenesis.

Molecular Weight: 47.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.

UniProt: Q8WZ75