ROBO4 Protein (Fc Tag,His tag)

Catalog No. ABIN7535301

Product Details

Target: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ROBO4 protein is labelled with Fc Tag,His tag.

Purpose: Recombinant Human ROBO4 Protein

Sequence: QDSPPQILVH PQDQLFQGPG PARMSCQASG QPPPTIRWLL NGQPLSMVPP DPHHLLPDGT LLLLQPPARG HAHDGQALST DLGVYTCEAS NRLGTAVSRG ARLSVAVLRE DFQIQPRDMV AVVGEQFTLE CGPPWGHPEP TVSWWKDGKP LALQPGRHTV SGGSLLMARA EKSDEGTYMC VATNSAGHRE SRAARVSIQE PQDYTEPVEL LAVRIQLENV TLLNPDPAEG PKPRPAVWLS WKVSGPAAPA QSYTALFRTQ TAPGGQGAPW AEELLAGWQS AELGGLHWGQ DYEFKVRPSS GRARGPDSNV LLLRLPEKVP SAPPQEVTLK PGNGTVFVSW VPPPAENHNG IIRGYQVWSL GNTSLPPANW TVVGEQTQLE IATHMPGSYC VQVAAVTGAG AGEPSRPVCL LLEQAMERAT QEPSEHGPWT LEQLRATLKR

Specificity: Gln28-Arg467

Purity: > 95 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for long term.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: ROBO4 (Roundabout, Axon Guidance Receptor, Homolog 4 (ROBO4))

Alternative Name: ROBO4 (ROBO4 Products)

Synonyms: ECSM4 Protein, MRB Protein, 1200012D01Rik Protein, AI593217 Protein, sc:d0823 Protein, wu:fa16f09 Protein, roundabout guidance receptor 4 Protein, roundabout, axon guidance receptor, homolog 4 (Drosophila) Protein, ROBO4 Protein, Robo4 Protein, robo4 Protein

Background:

Description: Roundabout homolog 4, also known as magic roundabout and ROBO4 is a member of the immunoglobulin superfamily and ROBO family. ROBO4 is specifically expressed in endothelial cells. It is expressed at sites of angiogenesis in different tumor types. ROBO4 contains two fibronectin type-III domains and two Ig-like C2-type (immunoglobulin-like) domains. ROBO4 is the fourth identified member of the roundabout receptor family. It is the only Robo family member expressed in primary endothelial cells and that application of Slit inhibits their migration. ROBO4 is predominantly expressed in embryonic or tumor vascular endothelium and is considered important for vascular development and as a candidate tumor endothelial marker. ROBO4 is a bona fide member of the Robo family and may provide a repulsive cue to migrating endothelial cells during vascular development. ROBO4 is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. Activating ROBO4 may have broad therapeutic application in diseases characterized by excessive angiogenesis and/or vascular leak.

Name: ROBO4,ECSM4,MRB

Gene ID: 54538

UniProt: Q8WZ75