RSPO1 Protein (AA 31-263) (His tag)
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- Target See all RSPO1 Proteins
- RSPO1 (R-Spondin 1 (RSPO1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 31-263
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Origin
- Human
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Source
- CHO Cells
- Purification tag / Conjugate
- This RSPO1 protein is labelled with His tag.
- Purpose
- Human R spondin 1/RSPO1 Protein
- Sequence
- Arg31-Ala263
- Characteristics
- Recombinant Human R spondin 1/RSPO1 Protein is expressed from CHO with His tag at the C-Terminus.It contains Arg31-Ala263.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 0.05 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human R spondin 1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-R spondin 1 Antibody, hFc Tag with the EC50 of 7.1ng/ml determined by ELISA. See testing image for detail.
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R-Spondin 1 (RSPO1) (AA 21-265) protein (His tag)
RSPO1 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
R-Spondin 1 (RSPO1) (AA 21-146) protein (His tag)RSPO1 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
R-Spondin 1 (RSPO1) (AA 21-146) protein (Fc Tag)RSPO1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
R-Spondin 1 (RSPO1) (AA 21-263) protein (His tag)RSPO1 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
R-Spondin 1 (RSPO1) (Active) protein (His tag)RSPO1 Origin: Mouse Host: CHO Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- RSPO1 (R-Spondin 1 (RSPO1))
- Alternative Name
- R spondin 1 (RSPO1 Products)
- Background
- The R-spondins are members of a superfamily of thrombospondin type 1 repeat (TSR-1)-containing proteins. The prototype member (discovered in 1971) was isolated from platelets that had been stimulated with thrombin, and was therefore designated 'thrombin-sensitive protein.
- Molecular Weight
- 27.3 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result.
- UniProt
- Q2MKA7
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