SEMA7A Protein (AA 45-648) (His tag)

Catalog No. ABIN7275564

This Recombinant SEMA7A protein is produced in HEK-293 Cells.

Quick Overview for SEMA7A Protein (AA 45-648) (His tag) (ABIN7275564)

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 45-648

Purification tag / Conjugate: This SEMA7A protein is labelled with His tag.

Purpose: Human SEMA7A Protein

Sequence: Gln45-Ala648

Characteristics: Recombinant Human SEMA7A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ala648.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human SEMA7A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Alternative Name: SEMA7A

Background: Semaphorin7A (Sema7A) plays an important role in the immunoregulation of the brain.Sema7A is upregulated in the epileptic brain and plays a potential role in the regulation of seizure activity in PTZ-kindled epileptic rats, which may be related to neuroinflammation. Sema7A promotes the inflammatory cytokines TNF-α and IL-6 as well as the growth of mossy fibers through the ERK pathway, suggesting that Sema7A may promote seizures by increasing neuroinflammation and activating pathological neural circuits. Sema7A plays a critical role in epilepsy and could be a potential therapeutic target for this neurological disorder.

Molecular Weight: 69.5 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Bis-Tris PAGE result.

UniProt: O75326

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size