SEMA3A Protein (AA 21-772) (His tag)

Catalog No. ABIN7275567

This Recombinant SEMA3A protein is produced in HEK-293 Cells.

Quick Overview for SEMA3A Protein (AA 21-772) (His tag) (ABIN7275567)

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 21-772

Purification tag / Conjugate: This SEMA3A protein is labelled with His tag.

Purpose: Mouse SEMA3A/Semaphorin-3A Protein

Sequence: Tyr21-Val772

Characteristics: Recombinant Mouse SEMA3A/Semaphorin-3A Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Tyr21-Val772.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Mouse SEMA3A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Mouse SEMA3A is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22 μm filtered solution in PBS, 200 mM L-Argine ( pH 7.4).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SEMA3A (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3A (SEMA3A))

Alternative Name: SEMA3A

Background: The CRMP proteins were originally identified as mediators of Sema3A signaling and neuronal differentiation. Much has been learned about the mechanism by which CRMPs regulate cellular responses to Sema3A.The secreted protein Sema3A (collapsin-1) was the first identified vertebrate semaphorin. Sema3A acts primarily as a repulsive axon guidance cue, and can cause a dramatic collapse of the growth cone lamellipodium.Neuropilin-1 (NP1) and members of the class A plexins (PlexA) form a Sema3A receptor complex, with NP1 serving as a high-affinity ligand binding partner, and PlexA transducing the signal into the cell via its large intracellular domain.

Molecular Weight: 86.6 kDa. Due to furin cleavage site, the protein migrates to 65-70 kDa based on Bis-Tris PAGE result.

UniProt: O08665

Pathways: Regulation of Cell Size