Sonic Hedgehog Protein (SHH) (AA 24-197)
Quick Overview for Sonic Hedgehog Protein (SHH) (AA 24-197) (ABIN7275581)
Target
See all Sonic Hedgehog (SHH) ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 24-197
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Purpose
- Human Sonic Hedgehog (Shh) Protein
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Sequence
- Cys24-Gly197
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Characteristics
- Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 0.05 EU per μg by the LAL method.
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Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) protein
SHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) proteinSHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Mouse Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) proteinSHH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) protein (His tag)SHH Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE SDS
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
Sonic Hedgehog (SHH) proteinSHH Origin: Human Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS, 300 mM NaCl ( pH 7.4). Normally 8 % trehalose / 8 % mannitol is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- Sonic Hedgehog (SHH)
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Alternative Name
- Sonic Hedgehog
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Background
- Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.
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Molecular Weight
- 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Tris-Bis PAGE result.
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UniProt
- Q15465
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Pathways
- Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Target
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