SIGLEC15 Protein (AA 60-322) (Biotin,His-Avi Tag)

Catalog No. ABIN7275585

This Recombinant SIGLEC15 protein is produced in HEK-293 Cells.

Quick Overview for SIGLEC15 Protein (AA 60-322) (Biotin,His-Avi Tag) (ABIN7275585)

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 60-322

Purification tag / Conjugate: This SIGLEC15 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Cynomolgus Siglec-15/CD33L3 Protein

Sequence: Met60-Thr322

Characteristics: Recombinant Biotinylated Cynomolgus Siglec-15/CD33L3 Protein is expressed from HEK293 with His and Avi tag at the C-Terminus.It contains Met60-Thr322.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Cynomolgus Siglec-15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-15 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).,Biotinylated Cynomolgus Siglec-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Cynomolgus Siglec-15 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in 20 mM PB, 500 mM NaCl, 0.1M L-arginine ( pH 6.0).

Storage: -80 °C

Storage Comment: Valid for 12 months from date of receipt when stored at -80°C.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Alternative Name: Siglec-15

Background: Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-15 consists of a 244 amino acid extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 is a potential therapeutic target for osteoporosis and plays a conserved regulatory role in the immune system of vertebrates.

Molecular Weight: 29.2 kDa. Due to glycosylation, the protein migratesto 33-43 kDa based on Bis-Tris PAGE result.

NCBI Accession: XP_005586866