SIGLEC15 Protein (His tag)

Catalog No. ABIN7321020

Product Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus

Source: Human Cells

Purification tag / Conjugate: This SIGLEC15 protein is labelled with His tag.

Purpose: Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

Sequence: Phe20-Thr263

Characteristics: Recombinant Cynomolgus Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus.

Purity: >95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: mmobilized Cynomolgus Siglec-15-His(Cat#PKSQ050096) at 2μg/ml (100 μl/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 125 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, 150 mMNaCl, 0.3 % Chaps, 5 % Trehalose, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: SIGLEC15 (Sialic Acid Binding Ig-Like Lectin 15 (SIGLEC15))

Alternative Name: Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (SIGLEC15 Products)

Synonyms: RGD1565948 Protein, CD33L3 Protein, HsT1361 Protein, SIGLEC-15 Protein, Cd33l3 Protein, EG620235 Protein, SIGLEC-I Protein, sialic acid binding Ig-like lectin 15 Protein, sialic acid binding Ig like lectin 15 Protein, Siglec15 Protein, SIGLEC15 Protein

Background:

Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Synonym: Angiopoietin-related protein 4, 425O18-1, Angiopoietin-like protein 4, Fasting-induced adipose factor, Hepatic fibrinogen/angiopoietin-related protein, HFARP, Secreted protein Bk89, Angptl4, Farp, Fiaf, Ng27

Molecular Weight: 27.1 kDa

UniProt: A0A2K5UY47