MAG Protein (AA 20-516) (Biotin,His-Avi Tag)

Catalog No. ABIN7275610

This Recombinant MAG protein is expressed in HEK-293 Cells.

Quick Overview for MAG Protein (AA 20-516) (Biotin,His-Avi Tag) (ABIN7275610)

Target: MAG (Myelin Associated Glycoprotein (MAG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 20-516

Purification tag / Conjugate: This MAG protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human Siglec-4a/MAG Protein

Sequence: Gly20-Pro516

Characteristics: Recombinant Biotinylated Human Siglec-4a/MAG Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly20-Pro516.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Siglec-4a Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human Siglec-4a, His Tag with the EC50 of 31.1ng/ml determined by ELISA.,Biotinylated Human Siglec-4a on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human Siglec-4a is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: Siglec-4a

Background: Siglec-4a, also known as Myelin-Associated Glycoprotein (MAG), is a type I transmembrane glycoprotein belonging to the Siglec family, a subgroup of the Ig superfamily. Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2 (By similarity).

Molecular Weight: 57.6 kDa. Due to glycosylation, the protein migrates to 78-85 kDa based on Bis-Tris PAGE result.

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway