SIRP AlphaV2 (AA 31-369) (Active) protein (His tag)

Catalog No. ABIN7275653

Recombinant SIRP AlphaV2 protein expressed in HEK-293 Cells.

Quick Overview for SIRP AlphaV2 (AA 31-369) (Active) protein (His tag) (ABIN7275653)

Target: SIRP AlphaV2

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-369

Purification tag / Conjugate: His tag

Purpose: Human SIRP alpha V2/CD172a Protein

Sequence: Glu31-Arg369

Characteristics: Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg369.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human SIRP alpha V2, His Tag captured on Protein A Chip via Anti-His Antibody can bind Anti-SIRP alpha Ab., hFc Tag with an affinity constant of 9.11nM as determined in SPR assay (Biacore T200).,Immobilized Human SIRP alpha V2, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 6.7ng/ml determined by ELISA (QC Test).,Immobilized Human SIRP alpha V2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-SIRP alpha Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.,Human SIRP alpha V2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Serial dilutions of Anti-SIRP alpha Antibody, hFc Tag were added into Human SIRP alpha V2, His Tag : Biotinylated Human CD47 Protein, His Tag binding reactioins.The half maximal inhibitiory concentration (IC50) is 54.14ng/ml.,The purity of Human SIRP alpha V2 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 24 months

Target Details

Target: SIRP AlphaV2

Alternative Name: SIRP alpha V2

Background: Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Molecular Weight: 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.