SIRP AlphaV2 (AA 31-369) protein (His tag)

Catalog No. ABIN7275653

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for SIRP AlphaV2 (AA 31-369) protein (His tag) (ABIN7275653)

Target: SIRP AlphaV2

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 31-369

Purification tag / Conjugate: His tag

Purpose: Human SIRP alpha V2/CD172a Protein

Sequence: Glu31-Arg369

Characteristics: Recombinant Human SIRP alpha V2/CD172a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu31-Arg369.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SIRP alpha V2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 0.15μg/ml determined by ELISA. The affinity constant of 9.11nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIRP AlphaV2

Alternative Name: SIRP alpha V2

Background: Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.

Molecular Weight: 38 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.