SIRP-beta Protein (AA 30-369) (Biotin,His-Avi Tag)

Catalog No. ABIN7275657

This Recombinant SIRP-beta protein is produced in HEK-293 Cells.

Quick Overview for SIRP-beta Protein (AA 30-369) (Biotin,His-Avi Tag) (ABIN7275657)

Target: SIRP-beta (SIRPB1A) (Signal-Regulatory Protein beta 1A (SIRPB1A))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-369

Purification tag / Conjugate: This SIRP-beta protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human SIRP Beta/CD172b Protein

Sequence: Glu30-Ala369

Characteristics: Recombinant Biotinylated Human SIRP Beta/CD172b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu30-Ala369.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human SIRP Beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human SIRP Beta is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIRP-beta (SIRPB1A) (Signal-Regulatory Protein beta 1A (SIRPB1A))

Alternative Name: SIRP Beta

Background: SIRP beta 1 is a type I transmembrane protein belonging to the SIRP family within the Ig superfamily. Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains.SIRP beta 1 is immunoglobulin-like cell surface receptor involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. Participates also in the recruitment of tyrosine kinase SYK. Triggers activation of myeloid cells when associated with TYROBP.

Molecular Weight: 40 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

UniProt: O00241