SIRPB1 Protein (AA 30-371) (His-Avi Tag)

Catalog No. ABIN7275660

This Recombinant SIRPB1 protein is produced in HEK-293 Cells.

Quick Overview for SIRPB1 Protein (AA 30-371) (His-Avi Tag) (ABIN7275660)

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 30-371

Purification tag / Conjugate: This SIRPB1 protein is labelled with His-Avi Tag.

Purpose: Non-biotinylated Human SIRP Beta 1 isoform 3 Protein

Sequence: Glu30-Leu371

Characteristics: Recombinant Human SIRP Beta 1 isoform 3 Proteinis expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu30-Leu371.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human SIRP Beta 1 isoform 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human SIRP Beta 1 isoform 3 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SIRPB1 (Signal-Regulatory Protein beta 1 (SIRPB1))

Alternative Name: SIRP Beta 1

Background: Signal regulatory protein β1 (SIRPB1) is a signal regulatory protein member of the immunoglobulin superfamily and is capable of modulating receptor tyrosine kinase-coupled signaling. Copy number variations at the SIRPB1 locus were previously reported to associate with prostate cancer aggressiveness in patients.SIRPB1 gene amplification and overexpression were detected in prostate cancer specimens. The knockdown of SIRPB1 significantly suppressed cell growth in colony formation assays and cell mobility.

Molecular Weight: 39.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

UniProt: Q5TFQ8