Transferrin Receptor Protein (AA 89-760) (His-Avi Tag,Biotin)

Catalog No. ABIN7275703

This Recombinant Transferrin Receptor protein is expressed in HEK-293 Cells.

Quick Overview for Transferrin Receptor Protein (AA 89-760) (His-Avi Tag,Biotin) (ABIN7275703)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 89-760

Purification tag / Conjugate: This Transferrin Receptor protein is labelled with His-Avi Tag,Biotin.

Purpose: Biotinylated Human Transferrin R/CD71 Protein

Sequence: Cys89-Phe760

Characteristics: Recombinant Biotinylated Human Transferrin R/CD71 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Cys89-Phe760.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Transferrin R Antibody at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin R, His Tag with the EC50 of 41.4ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 24 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: Transferrin R

Background: The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.

Molecular Weight: 77.9 kDa. Due to glycosylation, the protein migrates to 80-85 kDa based on Tris-Bis PAGE result.

UniProt: P02786

Pathways: Transition Metal Ion Homeostasis