Transferrin Receptor Protein (AA 89-760) (Fc Tag)
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- Target See all Transferrin Receptor (TFRC) Proteins
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 89-760
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Transferrin Receptor protein is labelled with Fc Tag.
- Purpose
- Human Transferrin R/CD71 Protein
- Sequence
- Cys89-Phe760
- Characteristics
- Recombinant Human Transferrin R/CD71 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys89-Phe760.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Transferrin R, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Transferrin R Antibody, hFc Tag with the EC50 of 25.6ng/ml determined by ELISA. The affinity constant of 0.1μM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
- Alternative Name
- Transferrin R (TFRC Products)
- Synonyms
- Trfr Protein, LOC100219280 Protein, tfr Protein, cd71 Protein, tfr1 Protein, trfr Protein, CD71 Protein, T9 Protein, TFR Protein, TFR1 Protein, TR Protein, TRFR Protein, p90 Protein, 2610028K12Rik Protein, AI195355 Protein, AI426448 Protein, AU015758 Protein, E430033M20Rik Protein, Mtvr-1 Protein, Mtvr1 Protein, transferrin receptor Protein, TFRC Protein, Tfrc Protein, tfrc Protein
- Background
- The transferrin receptor (CD71) is known as a receptor for IgA1 on mesangial cells. The transcript and protein expression of glomerular CD71 was determined by real-time polymerase chain reaction and immunohistochemistry. Human mesangial cells (HMCs) were treated with sera from IgAN patients and expression levels of CD71 and inflammatory cytokine markers were compared according to disease status.
- Molecular Weight
- 101.8 kDa. Due to glycosylation, the protein migrates to 105-115 kDa based on Tris-Bis PAGE result.
- Pathways
- Transition Metal Ion Homeostasis
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